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westChallenging Bone Samples Successfully Profiled Using RapidHit ID System

Jun 6, 2024 15:06 PM - Jun 6, 2024 15:06 PM, Monica Rockswold, Biological Sciences, Section Presentation

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When mass disasters occur, the number of victims increases rapidly prompting the need for disaster victim identification (DVI). Realistically, victim bone conditions are not pristine resulting in complex sample collection and processing. The Applied Biosystems RapidHITTM ID (RHID) system is already used in the field for human identification. This system can generate an STR human profile within compatible with CODIS within ~90 minutes, a fraction of traditional laboratory analysis time. Therefore, its use is vital in mass disaster situations. Using a standard drill, common drill bits, a vise, and an analytical scale; sample collection from bone can be fast and simplified. Direct RHID analysis was demonstrated to be successful on ~30mg of bone fragments maintained in an airconditioned laboratory setting as well as exposed to the elements of a Florida summer for up to 24 days. Bone buried in the earth still produced an interpretable profile after a 7-day exposure to organisms found in the soil collected from outside. The bone submerged in a local pond only generated an interpretable profile after the first day, failing on the second time point of 4 days. The study conducted at GFJC has further shown that coupling the RHID with the Applied Biosystems AutoMate ExpressTM enhances profiling results for the earth and water compromised bone. The 20uL eluate obtained from sample extraction and purification successfully generated interpretable results for the bone buried in the to earth after a 24-day exposure. Moreover, the AutoMate successfully extended the sample collection timeframe of the bone submerged in a pond from 1 day to a full week. By combining the strengths of a drilling method and existing automated preprocessing with the already proven rapid instrumentation, the capabilities of quickly gathering and analyzing samples to generate interpretable human forensics profiles from mass disaster situations have been expanded.