DNA found in crime scene evidence may be limited. The ability to analyze samples for DNA typing and retesting is restricted by the total DNA with subsequent analyses dependent on how much DNA remains. This may preclude performing any additional testing that could lead to important probative information. By utilizing the waste product that is typically discarded, additional testing can be performed that would normally be disregarded due to a lack of DNA sample. One important additional test is the identification of body fluids. Body fluid identification is important in forensic cases where an argument is present regarding the source of the DNA. For example, the suspect may claim the sample is innocent saliva or epithelial tissues, however, using epigenetic analysis, the actual identity of the sample may be revealed to be semen. This can be completed by analyzing the epigenetic patterns of body fluids which can differ based on specific methylation patterns at CpG sites, revealed by performing bisulfite conversion on the extracted DNA. This method reveals which cytosines are methylated which can be used to calculate the percent methylation at previously documented CpG sites. These patterns are unique for each body fluid and can be used to identify them from the original DNA sample. The aim of this study was to investigate DNA recovery from the extraction waste for body fluid identification. Four body fluids: buccal cells, vaginal epithelia, blood and semen were evaluated from 24 donors. An optimized purification protocol was developed for the extraction waste in triplicates for each body fluid. DNA concentrations, from the extracts and their corresponding purified wastes, were quantified using an in-house Alu intercalating dye-qPCR based assay. The extracts and waste products were then exposed to bisulfite conversion to differentiate methylated from unmethylated cytosines. Four epigenetic markers previously identified in our laboratory: BCAS4 (buccal cells), VE...